Detailed view for Tb927.7.4000

Basic information

TDR Targets ID: 11972
Trypanosoma brucei, glutathione synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9595 | Length (AA): 555 | MW (Da): 61304 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03199   Eukaryotic glutathione synthase
PF03917   Eukaryotic glutathione synthase, ATP binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016874   ligase activity  
GO:0005524   ATP binding  
GO:0004363   glutathione synthase activity  
GO:0003824   catalytic activity  
GO:0006750   glutathione biosynthetic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 555 2wyo (A) 1 555 99.99 0 1 2.0783 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2WYO:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128131)

Species Accession Gene Product
Arabidopsis thaliana AT5G27380   glutathione synthetase 2
Babesia bovis BBOV_IV004820   glutathione synthase, putative
Brugia malayi Bm1_05300   glutathione synthetase family protein
Candida albicans CaO19.6404   likely glutathione synthetase similar to S. cerevisiae GSH2 (YOL049W)
Candida albicans CaO19.13762   likely glutathione synthetase similar to S. cerevisiae GSH2 (YOL049W)
Caenorhabditis elegans CELE_M176.2   Protein GSS-1
Dictyostelium discoideum DDB_G0291756   glutathione synthase
Drosophila melanogaster Dmel_CG32495   CG32495 gene product from transcript CG32495-RD
Drosophila melanogaster Dmel_CG6835   Glutathione Synthetase
Echinococcus granulosus EgrG_000700600   glutathione synthetase
Echinococcus multilocularis EmuJ_000700600   glutathione synthetase
Giardia lamblia GL50803_15429   Glutathione synthetase, putative
Homo sapiens ENSG00000100983   glutathione synthetase
Leishmania braziliensis LbrM.14.0880   glutathione synthetase, putative
Leishmania donovani LdBPK_140970.1   glutathione synthetase, putative
Leishmania infantum LinJ.14.0970   glutathione synthetase, putative
Leishmania major LmjF.14.0910   glutathione synthetase, putative
Leishmania mexicana LmxM.14.0910   glutathione synthetase, putative
Loa Loa (eye worm) LOAG_07041   glutathione synthetase
Mus musculus ENSMUSG00000027610   glutathione synthetase
Oryza sativa 9272324   Os12g0528400
Oryza sativa 4351008   Os11g0642800
Oryza sativa 4351923   Os12g0263000
Plasmodium berghei PBANKA_1111800   glutathione synthetase
Plasmodium falciparum PF3D7_0512200   glutathione synthetase
Plasmodium knowlesi PKNH_0111100   glutathione synthetase, putative
Plasmodium knowlesi PKNH_1021400   glutathione synthetase, putative, pseudo
Plasmodium vivax PVX_080630   glutathione synthetase, putative
Plasmodium yoelii PY07248   putative glutathione synthetase
Saccharomyces cerevisiae YOL049W   glutathione synthase
Schistosoma japonicum Sjp_0014450   ko:K01920 glutathione synthase [EC6.3.2.3], putative
Schmidtea mediterranea mk4.001074.07   Glutathione synthetase
Trypanosoma brucei gambiense Tbg972.7.4490   glutathione synthetase, putative
Trypanosoma brucei Tb927.7.4000   glutathione synthetase, putative
Trypanosoma congolense TcIL3000_7_3190   glutathione synthetase, putative
Trypanosoma congolense TcIL3000_7_3200   Eukaryotic glutathione synthase, ATP binding domain containing protein, putative
Trypanosoma cruzi TcCLB.506659.30   glutathione synthetase, putative
Trypanosoma cruzi TcCLB.508865.10   glutathione synthetase, putative
Theileria parva TP01_0265   glutathione synthetase, putative
Theileria parva TP01_0264   glutathione synthetase, putative

Essentiality

Tb927.7.4000 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4000 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4000 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.4000 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4000 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_M176.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_M176.2 Caenorhabditis elegans larval arrest wormbase
CELE_M176.2 Caenorhabditis elegans slow growth wormbase
PBANKA_1111800 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

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User comments

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Gene identifier Tb927.7.4000 (Trypanosoma brucei), glutathione synthetase, putative
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